Decreased myocardial capillary density has been reported as an important histopathological feature associated with various heart disorders. Quantitative assessment of cardiac capillarization typically involves double immunostaining of cardiomyocytes (CMs) and capillaries in myocardial slices. In contrast, single immunostaining of basement membrane components is a straightforward approach to simultaneously label CMs and capillaries, presenting fewer challenges in background staining. However, subsequent image analysis always requires manual work in identifying and segmenting CMs and capillaries. Here, we developed an image analysis tool, AutoQC, to automatically identify and segment CMs and capillaries in immunofluorescence images of collagen type IV, a predominant basement membrane protein within the myocardium. In addition, commonly used capillarization-related measurements can be derived from segmentation masks. AutoQC features a weakly supervised instance segmentation algorithm by leveraging the power of a pre-trained segmentation model via prompt engineering. AutoQC outperformed YOLOv8-Seg, a state-of-the-art instance segmentation model, in both instance segmentation and capillarization assessment. Furthermore, the training of AutoQC required only a small dataset with bounding box annotations instead of pixel-wise annotations, leading to a reduced workload during network training. AutoQC provides an automated solution for quantifying cardiac capillarization in basement-membrane-immunostained myocardial slices, eliminating the need for manual image analysis once it is trained.
相關內容
Background: Pain assessment in individuals with neurological conditions, especially those with limited self-report ability and altered facial expressions, presents challenges. Existing measures, relying on direct observation by caregivers, lack sensitivity and specificity. In cerebral palsy, pain is a common comorbidity and a reliable evaluation protocol is crucial. Thus, having an automatic system that recognizes facial expressions could be of enormous help when diagnosing pain in this type of patient. Objectives: 1) to build a dataset of facial pain expressions in individuals with cerebral palsy, and 2) to develop an automated facial recognition system based on deep learning for pain assessment addressed to this population. Methods: Ten neural networks were trained on three pain image databases, including the UNBC-McMaster Shoulder Pain Expression Archive Database, the Multimodal Intensity Pain Dataset, and the Delaware Pain Database. Additionally, a curated dataset (CPPAIN) was created, consisting of 109 preprocessed facial pain expression images from individuals with cerebral palsy, categorized by two physiotherapists using the Facial Action Coding System observational scale. Results: InceptionV3 exhibited promising performance on the CP-PAIN dataset, achieving an accuracy of 62.67% and an F1 score of 61.12%. Explainable artificial intelligence techniques revealed consistent essential features for pain identification across models. Conclusion: This study demonstrates the potential of deep learning models for robust pain detection in populations with neurological conditions and communication disabilities. The creation of a larger dataset specific to cerebral palsy would further enhance model accuracy, offering a valuable tool for discerning subtle and idiosyncratic pain expressions. The insights gained could extend to other complex neurological conditions.
We study the problem of stereo singing voice cancellation, a subtask of music source separation, whose goal is to estimate an instrumental background from a stereo mix. We explore how to achieve performance similar to large state-of-the-art source separation networks starting from a small, efficient model for real-time speech separation. Such a model is useful when memory and compute are limited and singing voice processing has to run with limited look-ahead. In practice, this is realised by adapting an existing mono model to handle stereo input. Improvements in quality are obtained by tuning model parameters and expanding the training set. Moreover, we highlight the benefits a stereo model brings by introducing a new metric which detects attenuation inconsistencies between channels. Our approach is evaluated using objective offline metrics and a large-scale MUSHRA trial, confirming the effectiveness of our techniques in stringent listening tests.
In this study, a finite deformation phase-field formulation is developed to investigate the effect of hygrothermal conditions on the viscoelastic-viscoplastic fracture behavior of epoxy nanocomposites under cyclic loading. The formulation incorporates a definition of the Helmholtz free energy, which considers the effect of nanoparticles, moisture content, and temperature. The free energy is additively decomposed into a deviatoric equilibrium, a deviatoric non-equilibrium, and a volumetric contribution, with distinct definitions for tension and compression. The proposed derivation offers a realistic modeling of damage and viscoplasticity mechanisms in the nanocomposites by coupling the phase-field damage model with a modified crack driving force and a viscoelastic-viscoplastic model. Numerical simulations are conducted to study the cyclic force-displacement response of both dry and saturated boehmite nanoparticle (BNP)/epoxy samples, considering BNP contents and temperature. Comparing numerical results with experimental data shows good agreement at various BNP contents. In addition, the predictive capability of the phase-field model is evaluated through simulations of single-edge notched nanocomposite plates subjected to monolithic tensile and shear loading.
Recently, a family of unconventional integrators for ODEs with polynomial vector fields was proposed, based on the polarization of vector fields. The simplest instance is the by now famous Kahan discretization for quadratic vector fields. All these integrators seem to possess remarkable conservation properties. In particular, it has been proved that, when the underlying ODE is Hamiltonian, its polarization discretization possesses an integral of motion and an invariant volume form. In this note, we propose a new algebraic approach to derivation of the integrals of motion for polarization discretizations.
During multiple testing, researchers often adjust their alpha level to control the familywise error rate for a statistical inference about a joint union alternative hypothesis (e.g., "H1 or H2"). However, in some cases, they do not make this inference and instead make separate inferences about each of the individual hypotheses that comprise the joint hypothesis (e.g., H1 and H2). For example, a researcher might use a Bonferroni correction to adjust their alpha level from the conventional level of 0.050 to 0.025 when testing H1 and H2, find a significant result for H1 (p < 0.025) and not for H2 (p > .0.025), and so claim support for H1 and not for H2. However, these separate individual inferences do not require an alpha adjustment. Only a statistical inference about the union alternative hypothesis "H1 or H2" requires an alpha adjustment because it is based on "at least one" significant result among the two tests, and so it depends on the familywise error rate. When a researcher corrects their alpha level during multiple testing but does not make an inference about the union alternative hypothesis, their correction is redundant. In the present article, I discuss this redundant correction problem, including its associated loss of statistical power and its potential causes vis-\`a-vis error rate confusions and the alpha adjustment ritual. I also provide three illustrations of redundant corrections from recent psychology studies. I conclude that redundant corrections represent a symptom of statisticism, and I call for a more nuanced and context-specific approach to multiple testing corrections.
The need to Fourier transform data sets with irregular sampling is shared by various domains of science. This is the case for example in astronomy or sismology. Iterative methods have been developed that allow to reach approximate solutions. Here an exact solution to the problem for band-limited periodic signals is presented. The exact spectrum can be deduced from the spectrum of the non-equispaced data through the inversion of a Toeplitz matrix. The result applies to data of any dimension. This method also provides an excellent approximation for non-periodic band-limit signals. The method allows to reach very high dynamic ranges ($10^{13}$ with double-float precision) which depend on the regularity of the samples.
Methods for estimating heterogeneous treatment effects (HTE) from observational data have largely focused on continuous or binary outcomes, with less attention paid to survival outcomes and almost none to settings with competing risks. In this work, we develop censoring unbiased transformations (CUTs) for survival outcomes both with and without competing risks.After converting time-to-event outcomes using these CUTs, direct application of HTE learners for continuous outcomes yields consistent estimates of heterogeneous cumulative incidence effects, total effects, and separable direct effects. Our CUTs enable application of a much larger set of state of the art HTE learners for censored outcomes than had previously been available, especially in competing risks settings. We provide generic model-free learner-specific oracle inequalities bounding the finite-sample excess risk. The oracle efficiency results depend on the oracle selector and estimated nuisance functions from all steps involved in the transformation. We demonstrate the empirical performance of the proposed methods in simulation studies.
Dynamical systems across the sciences, from electrical circuits to ecological networks, undergo qualitative and often catastrophic changes in behavior, called bifurcations, when their underlying parameters cross a threshold. Existing methods predict oncoming catastrophes in individual systems but are primarily time-series-based and struggle both to categorize qualitative dynamical regimes across diverse systems and to generalize to real data. To address this challenge, we propose a data-driven, physically-informed deep-learning framework for classifying dynamical regimes and characterizing bifurcation boundaries based on the extraction of topologically invariant features. We focus on the paradigmatic case of the supercritical Hopf bifurcation, which is used to model periodic dynamics across a wide range of applications. Our convolutional attention method is trained with data augmentations that encourage the learning of topological invariants which can be used to detect bifurcation boundaries in unseen systems and to design models of biological systems like oscillatory gene regulatory networks. We further demonstrate our method's use in analyzing real data by recovering distinct proliferation and differentiation dynamics along pancreatic endocrinogenesis trajectory in gene expression space based on single-cell data. Our method provides valuable insights into the qualitative, long-term behavior of a wide range of dynamical systems, and can detect bifurcations or catastrophic transitions in large-scale physical and biological systems.
Semivariance is a measure of the dispersion of all observations that fall above the mean or target value of a random variable and it plays an important role in life-length, actuarial and income studies. In this paper, we develop a new non-parametric test for equality of upper semi-variance. We use the U-statistic theory to derive the test statistic and then study the asymptotic properties of the test statistic. We also develop a jackknife empirical likelihood (JEL) ratio test for equality of upper Semivariance. Extensive Monte Carlo simulation studies are carried out to validate the performance of the proposed JEL-based test. We illustrate the test procedure using real data.
Exponential families are statistical models which are the workhorses in statistics, information theory, and machine learning among others. An exponential family can either be normalized subtractively by its cumulant or free energy function or equivalently normalized divisively by its partition function. Both subtractive and divisive normalizers are strictly convex and smooth functions inducing pairs of Bregman and Jensen divergences. It is well-known that skewed Bhattacharryya distances between probability densities of an exponential family amounts to skewed Jensen divergences induced by the cumulant function between their corresponding natural parameters, and in limit cases that the sided Kullback-Leibler divergences amount to reverse-sided Bregman divergences. In this paper, we first show that the $\alpha$-divergences between unnormalized densities of an exponential family amounts to scaled $\alpha$-skewed Jensen divergences induced by the partition function. We then show how comparative convexity with respect to a pair of quasi-arithmetic means allows to deform both convex functions and their arguments, and thereby define dually flat spaces with corresponding divergences when ordinary convexity is preserved.
小貼士
登錄享
相關主題
注冊地址： 北京市海淀區羊坊店路18號2幢3層301-191